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KMID : 0665220090220030456
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Korean Journal of Food and Nutrition
2009 Volume.22 No. 3 p.456 ~ p.462
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Extracts of Adlay, Barley and Rice Bran have Antioxidant Activity and Modulate Fatty Acid Metabolism in Adipocytes
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Park Tae-Sik
Lee Su-Yeon
Kim Hyeon-Jin
Kim Kyung-Tack
Kim Young-Jun
Jeong In-Hye
Do Wan-Nyeo
Lee Hye-Jeong
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Abstract
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Adlay, barley and rice bran were extracted using various concentrations of methanol(10% and 80%) and chloroform : methanol(2 : 1) to examine the biological activities of these raw grains. Extraction with 80% methanol resulted in high Vitamin C Equivalent Antioxidant Capacity(VCEAC), in the order of barley > rice bran > adlay, as determined by DPPH and ABTS assays. In addition, the extracts of adlay and rice bran showed high cellular antioxidant activity in HepG2 cells possibly due to the presence of polyphenol glycosides in these grains. We examined the expression of glucose/fatty acid metabolizing genes in differentiated 3T3-L1 adipocyte cells. Glut1 was downregulated after treatment with rice bran and no changes in the expression of Glut4 was observed. In contrast, genes involved in fatty acid metabolism, CD36 and aP2, were upregulated. Since these physiological changes were matched with peroxisome proliferator activating receptor (PPAR ) agonism, we suggest that the extracts from adlay, barley and rice bran may play preventive roles against aging and diabetes via antioxidant activity and increased uptake of fatty acids by adipocytes.
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KEYWORD
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antioxidant activity, fatty acid metabolism, PPAR, aging, diabetes
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